Peer-reviewed veterinary case report
A bi-cistronic, reporter-encoding bovine viral diarrhea virus applied in a new, effective diagnostic test.
- Journal:
- The Journal of general virology
- Year:
- 2014
- Authors:
- Gebauer, Mandy et al.
- Affiliation:
- Institute of Biochemistry and Biotechnology · Germany
Plain-English summary
Researchers have developed a new test to help detect infections caused by bovine viral diarrhea virus (BVDV), which can significantly affect cattle health and farming profits. They created a modified version of the virus that includes a special marker, making it easier to identify infected or vaccinated cattle. This new test is quick and straightforward, allowing for better monitoring of herds and helping with vaccination and eradication efforts. The goal is to improve how farmers can manage and protect their cattle from this virus.
Abstract
Infections with bovine viral diarrhea virus (BVDV) have a huge economic impact on cattle production and reproduction worldwide. A key factor for BVDV surveillance and eventual eradication is to efficiently detect infections and to monitor herd immunity. In this study, we generated a stable, bi-cistronic BVDV that encoded EGFP in addition to the viral proteins. Applying this recombinant virus, a new flow-cytometry-based virus neutralization test was established that enabled accurate and reliable detection of field-virus-infected and vaccinated animals. The test, which is simple and fast, is expected to support novel, effective screening procedures in eradication and vaccination programmes.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24760759/