Peer-reviewed veterinary case report
A bioluminescent probe for in vivo imaging of pyroglutamate aminopeptidase in a mouse model of inflammation.
- Journal:
- Bioorganic & medicinal chemistry letters
- Year:
- 2021
- Authors:
- Hu, Shilong et al.
- Affiliation:
- Department of Medicinal Chemistry · China
Abstract
Pyroglutamate aminopeptidase (PGP) specifically cleaves the peptide bond of pyroglutamic acid linked to the N-terminal end of a polypeptide or protein. Previous studies showed that PGP was associated with several physiological processes and diseases especially those involving inflammation. Utilizing a 'caging' strategy, we designed and synthesized a bioluminescence probe (PBL) with a limit-of-detection of 3.7 * 10 mU/mL. In vivo imaging in a mouse model of inflammatory liver disease revealed that the probe has excellent sensitivity and selectivity and provides a powerful tool for studying the physiological and pathological processes involving PGP.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/33882272/