Peer-reviewed veterinary case report
A cell line resistant to avian leukosis virus subgroup B infection.
- Journal:
- Poultry science
- Year:
- 2019
- Authors:
- Zhao, Zi Jun et al.
- Affiliation:
- College of Veterinary Medicine · China
- Species:
- bird
Abstract
The expression of env proteins that bind to viral cell receptors on avian leukosis virus (ALV)-susceptible cells can block ALV infection. In this study, we constructed a cell line (DF-1/B) by expressing the ALV-B env protein in DF-1 cells. PCR, immune fluorescence assay, Western blot, and immune electron microscopy results showed that the env gene can be stably expressed in DF-1cells and the env protein could be detected on the DF-1 cell membrane. An antiviral experiment concluded that the DF-1/B cell line could be resistant to 1 × 104 TCID50 ALV-B virus infection, but had no inhibitory effect on other subgroup ALV. This means that the DF-1/B cell line is specifically resistant to ALV-B and can be used as a tool for ALV-B diagnosis.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/31376350/