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Peer-reviewed veterinary case report

A comparison of assays for the detection ofin the feces of scouring calves.

Journal:
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Year:
2022
Authors:
Atwal, Harveen K et al.
Affiliation:
Animal Health Centre · Canada

Abstract

is a zoonotic, protozoan parasite that causes potentially life-threatening diarrhea in the host and can be transmitted via the fecal-oral route.can infect cattle and may be detected in their feces using a variety of tests. We compared the level of agreement, ease of procedure, and cost among PCR, lateral flow immunoassay, fluorescent antibody, and Kinyoun acid-fast stain direct smear tests. Over the course of 9 mo, 74 calf fecal samples were submitted and tested forusing all 4 tests. A Fleiss kappa value of 0.813 was obtained, indicating an excellent level of agreement among tests. Overall, the best test based on cost and ease of procedure was the Kinyoun acid-fast stain direct smear.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/35000499/