Peer-reviewed veterinary case report
A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle.
- Journal:
- Molecular and cellular probes
- Year:
- 2017
- Authors:
- Gomes, Jacinto et al.
- Affiliation:
- Unidade Estraté
Abstract
A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/28013043/