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Peer-reviewed veterinary case report

A first case of ehrlichiosis in a horse in Poland.

Journal:
DTW. Deutsche tierarztliche Wochenschrift
Year:
2009
Authors:
Adaszek, Łukasz et al.
Affiliation:
Department of Epizootiology and Infectious Diseases Clinic
Species:
horse

Plain-English summary

A horse in Poland was diagnosed with ehrlichiosis, a disease caused by a type of bacteria. The horse showed signs of fever, swollen joints, weakness, and unusual bleeding on its mucous membranes. Blood tests confirmed the presence of Ehrlichia bacteria, and a special examination of the blood showed typical signs of the infection. The horse was treated with an antibiotic called oxytetracycline for a week, which led to a gradual improvement in its condition. This case marks the first recorded instance of ehrlichiosis in a horse in Poland, and the treatment was successful.

Abstract

The study was aimed at determining the cause of a disease in a horse exhibiting symptoms of fever, joint effusion, weakness, and extravasations on the mucous membranes. Blood was drawn from the animal for haematological and biochemical molecular tests. The PCR technique revealed the presence of 16S RNA Ehrlichia spp. genetic material in the blood samples. DNA amplification by means of primers EHR 521 and EHR 747 gave a product with a volume of 247 bp.The sequence of the PCR product obtained showed a 97.6% similarity with a sequence of a fragment of 16S RNA Ehrlichia phagocytophila, gene number EU 090186 in the GenBank. A microscopic examination of blood smears demonstrated the presence in the neutrophiles of intracytoplasmatic structures characteristic for Ehrlichia.The treatment involved the intravenous application of oxytetracycline at a dose of 8 mg/kg of body mass for 7 days, and resulted in a gradual recovery. The positive results of the molecular test and the positive reaction to the therapy are an indication of the first case of ehrlichiosis in a horse in Poland as confirmed in laboratory test.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/19813450/