Peer-reviewed veterinary case report
A globotetraosylceramide (Gb₄) receptor-based ELISA for quantitative detection of Shiga toxin 2e.
- Journal:
- The Journal of veterinary medical science
- Year:
- 2015
- Authors:
- Togashi, Katsuhiro et al.
- Affiliation:
- Yamagata Integrated Agricultural Research Center Swine Experiment Station · Japan
Abstract
Currently, no simple assays are available for routine quantitative detection of Escherichia coli-produced Shiga toxin 2e (Stx2e) that causes porcine edema disease. Here, we present a novel quantitative detection method for Stx2e based on the measurement of Stx2e binding to the specific globotetraosylceramide (Gb4) receptor by ELISA (Gb4-ELISA). No cross-reactivity was found with the other Shiga toxins Stx1 and Stx2, indicating high specificity. When the recombinant Stx2e B subunit (Stx2eB) was used, the absorbance measured by Gb4-ELISA increased linearly with Stx2eB concentration in the range of 20-2,500 ng/ml. The Gb4-ELISA method can be easily performed, suggesting that it would be a useful diagnostic tool for porcine edema disease.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25787850/