Peer-reviewed veterinary case report
How to tell if your cattle have lumpy skin disease or just got
By Menasherow, Sophia et al.·Published in Journal of virological methods·2016·Kimron Veterinary Institute·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: A high-resolution melting (HRM) assay for the differentiation between Israeli field and Neethling vaccine lumpy skin disease viruses.
- Species:
- cattle
Plain-English summary
Lumpy skin disease (LSD) is a serious illness that affects cattle, especially in the Middle East, including Israel. This study focused on creating a new test that can tell the difference between cattle infected with the harmful virus and those that have been vaccinated. The new test looks for a specific part of the virus that is found in wild strains but not in the vaccine strain, allowing for a clear distinction. The results showed that cattle that were vaccinated but still got sick had symptoms similar to those caused by the vaccine itself. Overall, this new test is quicker, simpler, and just as reliable as older methods for identifying the virus.
Abstract
Lumpy skin disease (LSD) is a constant threat to the Middle East including the State of Israel. During vaccination programs it is essential for veterinary services and farmers to be able to distinguish between animals affected by the cattle-borne virulent viruses and vaccinated animals, subsequently affected by the vaccine strain. This study describes an improved high resolution-melting (HRM) test that exploits a 27 base pair (bp) fragment of the LSDV126 extracellular enveloped virion (EEV) gene that is present in field viruses but is absent from the Neethling vaccine strain. This difference leads to ∼0.5 °C melting point change in the HRM assay, when testing the quantitative PCR (qPCR) products generated from the virulent field viruses compared to the attenuated vaccine. By exploiting this difference, it could be shown using the newly developed HRM assay that virus isolated from vaccinated cattle that developed disease symptoms behave similarly to vaccine virus control, indicating that the vaccine virus can induce disease symptoms. This assay is not only in full agreement with the previously published PCR gradient and restriction fragment length polymorphism (RFLP) tests but it is faster with, fewer steps, cheaper and dependable.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/26902159/