Peer-reviewed veterinary case report
A high-throughput technique to quantify bacterial pathogens' virulence on the insect model Galleria mellonella.
- Journal:
- Journal of microbiological methods
- Year:
- 2018
- Authors:
- Parthuisot, Nathalie et al.
- Affiliation:
- É · France
Abstract
We combined spectrophotometry and an original statistical approach to infer bacteria virulence, using the lepidoptera Galleria mellonella as a host model. With this method, it is possible to use a microplate reader to automatize data collection on host survival on batches of 96 samples. The method also allows measurement of pathogen multiplication if GFP labelled bacterial strains are used.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/30071255/