Peer-reviewed veterinary case report
A method for isolating and culturing placental cells from failed early equine pregnancies.
- Journal:
- Placenta
- Year:
- 2016
- Authors:
- Rose, B V et al.
- Affiliation:
- Department of Comparative Biomedical Sciences · United Kingdom
- Species:
- horse
Abstract
Early pregnancy loss occurs in 6-10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14-65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26907389/