Peer-reviewed veterinary case report
A modified HIV challenge assay in mice by using luciferase-expressing vaccinia virus.
- Journal:
- Vaccine
- Year:
- 2006
- Authors:
- Shinoda, Kaori et al.
- Affiliation:
- Department of Molecular Biodefense Research · Japan
- Species:
- rodent
Abstract
In this study, we developed a simple and sensitive assay in mice for a challenge experiment by using a recombinant vaccinia virus dual-expressing antigen (HIV Env gp160) and firefly luciferase. This assay can detect the vaccine effect at real-time in vivo by using a small amount of mouse serum. The luciferase activity in mouse serum was in agreement with the viral titer in the ovary. This assay would be applicable as a challenge model for infectious diseases.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/16417954/