Peer-reviewed veterinary case report
How to test dogs for three common viral gut infections at once
By Deng, Xiaoyu et al.·Published in Archives of virology·2018·Institute of Special Economic Animal and Plant Sciences, China·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: A multiplex PCR method for the simultaneous detection of three viruses associated with canine viral enteric infections.
- Species:
- dog
Plain-English summary
A group of dogs with diarrhea were tested for three viruses that can cause intestinal infections: canine parvovirus, canine coronavirus, and canine adenovirus. Researchers developed a new test that can detect all three viruses at once, making it easier and quicker for vets to diagnose the problem. Out of 63 dogs tested, the new method matched the results of traditional testing perfectly, identifying the viruses with high accuracy. This new test could help vets diagnose viral enteritis more efficiently and accurately, leading to better treatment for affected dogs.
People also search for: dog diarrhea causes · canine parvovirus treatment · how to test for dog viruses
Abstract
The aim of this study was to establish a multiplex PCR (mPCR) method that can simultaneously detect canine parvovirus (CPV-2), canine coronavirus (CCoV) and canine adenovirus (CAV), thereby eliminating the need to detect these pathogens individually. Based on conserved regions in the genomes of these three viruses, the VP2 gene of CPV-2, the endoribonuclease nsp15 gene of CCoV, and the 52K gene of CAV were selected for primer design. The specificity of the mPCR results showed no amplification of canine distemper virus (CDV), canine parainfluenza virus (CPIV), or pseudorabies virus (PRV), indicating that the method had good specificity. A sensitivity test showed that the detection limit of the mPCR method was 1 × 10viral copies. A total of 63 rectal swabs from dogs with diarrheal symptoms were evaluated using mPCR and routine PCR. The ratio of positive samples to total samples for CPV-2, CCoV, and CAV was 55.6% (35/63) for mPCR and 55.6% (35/63) for routine PCR. Thirty-five positive samples were detected by both methods, for a coincidence ratio of 100%. This mPCR method can simultaneously detect CCoV (CCoV-II), CAV (CAV-1, CAV-2) and CPV-2 (CPV-2a, CPV-2b, CPV-2c), which are associated with viral enteritis, thereby providing an efficient, inexpensive, specific, and accurate new tool for clinical diagnosis and laboratory epidemiological investigations.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/29675651/