Peer-reviewed veterinary case report
A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay.
- Journal:
- Journal of virological methods
- Year:
- 2013
- Authors:
- Amer, H M et al.
- Affiliation:
- Department of Botany and Microbiology
Plain-English summary
Bovine coronavirus (BCoV) is a virus that can cause serious health issues in calves, like diarrhea, and can also affect adult cattle, leading to respiratory problems. Quickly diagnosing BCoV is important for reducing its impact on farms. Researchers have developed a new test called reverse transcription recombinase polymerase amplification (RT-RPA) that can detect BCoV in just 10 to 20 minutes, which is much faster than the traditional method known as real-time RT-PCR. This new test has been shown to be accurate and sensitive, and it doesn't confuse BCoV with other viruses that cause similar symptoms in cattle. Overall, the RT-RPA test is a promising option for diagnosing BCoV more quickly and easily, especially in field settings.
Abstract
Bovine coronavirus (BCoV) is an economically significant cause of calf scours and winter dysentery of adult cattle, and may induce respiratory tract infections in cattle of all ages. Early diagnosis of BCoV helps to diminish its burden on the dairy and beef industry. Real-time RT-PCR assay for the detection of BCoV has been described, but it is relatively expensive, requires well-equipped laboratories and is not suitable for on-site screening. A novel assay, using reverse transcription recombinase polymerase amplification (RT-RPA), for the detection of BCoV is developed. The BCoV RT-RPA was rapid (10-20 min) and has an analytical sensitivity of 19 molecules. No cross-reactivity with other viruses causing bovine gastrointestinal and/or respiratory infections was observed. The assay performance on clinical samples was validated by testing 16 fecal and 14 nasal swab specimens and compared to real-time RT-PCR. Both assays provided comparable results. The RT-RPA assay was significantly more rapid than the real-time RT-PCR assay. The BCoV RT-RPA constitutes a suitable accurate, sensitive and rapid alternative to the common measures used for BCoV diagnosis. In addition, the use of a portable fluorescence reading device extends its application potential to use in the field and point-of-care diagnosis.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23811231/