Peer-reviewed veterinary case report
Fast test to diagnose feline panleukopenia in cats
By Bakde, R A et al.·Published in Journal of the South African Veterinary Association·2024·Department of Veterinary Epidemiology and Preventive Medicine, India·View original on PubMed →
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Original publication title: A novel loop-mediated isothermal amplification (LAMP) assay to diagnose feline panleukopenia.
- Species:
- cat
Plain-English summary
A new test has been developed to quickly diagnose feline panleukopenia, a serious and contagious virus that affects cats. This test, called LAMP, can analyze fecal samples and provide results in about an hour, making it faster and potentially cheaper than traditional methods. It has shown to be very accurate, detecting the virus in 100% of positive samples and correctly identifying 90% of negative ones. This could help veterinarians start treatment sooner and control outbreaks more effectively.
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Abstract
, known commonly as feline panleukopenia virus (FPV) is a highly contagious and environmentally stable parvovirus of domestic as well as wild felids. A rapid and robust diagnostic tool will aid in implementing prompt treatment and control measures. A loop-mediated isothermal amplification (LAMP) as a point-of-care diagnostic tool for diagnosing feline panleukopenia was standardised using faecal samples of cats. The assay will reduce the cost and time required to diagnose feline panleukopenia. A set of two outer primers (F3 and B3) and two inner primers (FIP and BIP) were designed to target the viral polypeptide (VP2) gene of FPV. Optimisation of the LAMP reaction was done at 60 °C for one hour after an initial denaturation at 95 °C for five minutes. Visualisation of the result based on the addition of SYBR Green 1 dye offered an easy and reliable diagnosis. The detection limit of the standardised LAMP assay was as low as 1.25 ng/μl of the target DNA. Species specificity of the LAMP primers revealed no amplification of the non-target DNA of any other species except that of the canine parvovirus DNA template. DNA extracted from 100 PCR-positive and 20 PCR-negative faecal samples were subjected to the standardised assay and compared with PCR. Analysis of the results revealed that the LAMP assay was 100% sensitive and 90% specific compared to PCR. The LAMP assay could be a reliable tool for the point-of-care diagnosis of feline panleukopenia in limited resource settings.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/38533815/