Peer-reviewed veterinary case report
New PCR test improves diagnosis of calicivirus in cats
By Marsilio, Fulvio et al.·Published in Veterinary microbiology·2005·Department of Scienze Biomediche Comparate, Italy·View original on PubMed →
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Original publication title: A novel nested PCR for the diagnosis of calicivirus infections in the cat.
- Species:
- cat
Plain-English summary
A group of cats with respiratory problems were tested for feline calicivirus (FCV) using a new, more sensitive test called nested PCR. Out of 87 samples taken from their eyes and throats, 18 from the eyes and 23 from the throats tested positive for the virus. This new test proved to be better at detecting the virus compared to older methods. Using this improved test can help veterinarians diagnose FCV infections more accurately in cats showing respiratory symptoms.
People also search for: cat respiratory problems · feline calicivirus test · cat coughing treatment
Abstract
A novel nested PCR (nPCR) assay is reported on the diagnosis of the feline calicivirus (FCV) infection. The test was performed on 47 ocular and 40 pharyngeal swabs collected from 47 cats with respiratory syndrome; among the 87 samples examined, 18 ocular and 23 pharyngeal swabs were positive in nPCR. The nPCR sensitivity was compared to other diagnostic techniques such as virus isolation on cell culture and reverse transcriptase-polymerase chain reaction (RT-PCR). The nPCR was more sensitive than the virus isolation and RT-PCR; therefore, it can be used for calicivirosis diagnosis in cats.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/15607078/