Peer-reviewed veterinary case report
A novel pH-responsive electrochemiluminescence immunosensor for ALV-J detection based on hollow MnOencapsulating Ru(bpy)Cl.
- Journal:
- Biosensors & bioelectronics
- Year:
- 2018
- Authors:
- Liu, Chao et al.
- Affiliation:
- College of Chemistry and Material Science · China
- Species:
- bird
Abstract
A novel pH-responsive sandwich-type electrochemiluminescence immunosensor was successfully developed for the detection of avian leukosis virus subgroup J (ALV-J). The immunosensor consisted of graphene oxide functionalized with tannic acid (rGO-TA) and primary antibodies (Ab) as the main ALV-J sensing platform. For signal amplification, pH-responsive hollow MnO(hMnO) nanospheres encapsulating Ru(bpy)Cland carrying secondary antibodies (Ab) were employed. Under optimal conditions, the immunosensor exhibited a wide ALV-J detection range of 10to 10TCID/mL (TCID: 50% tissue culture infective dose) and a low detection limit of 10TCID/mL (S/N = 3). Importantly, the immunosensor showed high sensitivity, good reproducibility and excellent operational stability. Finally, the immunosensor was evaluated using a real avian serum sample containing ALV-J, with excellent results being achieved.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/30077130/