Peer-reviewed veterinary case report
A novel, simplified technique to amplify Eimeria (Coccidia: Apicomplexa) DNA from oocysts.
- Journal:
- The Journal of parasitology
- Year:
- 2015
- Authors:
- Gerhold, R W et al.
- Affiliation:
- Department of Poultry Science · United States
Abstract
A new method to amplify coccidia DNA by polymerase chain reaction (PCR) was developed by placing freeze-thawed oocysts in Ready-to-Go PCR bead tubes and using a 5-min initial heat denaturation step. Positive PCR reactions were found in 3 of 3 samples containing 20 or 50 oocysts; when ≤5 oocysts were used, 1 of 3 samples was positive. This technique shows potential for effectively and efficiently detecting and identifying oocysts from soil, feces, and other matter.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25019284/