A Numerical and Experimental Study on the Enrichment Performance of a Novel Multi-Physics Coupling Microchannel.

Abstract

The coupled method of inertial focusing and magnetic separation is effective for detecting and isolating circulating tumor cells (CTCs) from blood, wherein the design of a multi-physics coupled microfluidic device plays a critical role in the sorting efficiency. This paper presents a novel compact microfluidic device that combines inertial and magnetic forces for CTC separation. Using the finite element method, the effects of three major parameters (e.g., fluid velocity, particle properties, and magnetic field distribution) on sorting efficiency were comprehensively investigated and discussed. Simulated and experimental results demonstrate that the designed compact microfluidic device with coupled physical fields achieves high separation purity (>98%) for CTCs larger than 19 μm in diameter over a wide range of parameters, such as a fluid velocity greater than 3.5 × 10^-8 m³/s, a remanent flux density between 1.08 T and 1.28 T, and the position of the magnet ranging from 2.5 mm to 4 mm.