Peer-reviewed veterinary case report
New test for diagnosing caseous lymphadenitis in goats and sheep
By Barral, Thiago Doria et al.·Published in Microbial biotechnology·2019·Laborató·View original on PubMed →
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Original publication title: A panel of recombinant proteins for the serodiagnosis of caseous lymphadenitis in goats and sheep.
Plain-English summary
Caseous lymphadenitis (CLA) is a disease that affects goats and sheep, causing lumps in their lymph nodes and can lead to significant financial losses for farmers. The disease is caused by a bacterium called Corynebacterium pseudotuberculosis, but current tests for it can sometimes give incorrect results. Researchers developed a new blood test called an ELISA that can more accurately detect the disease in these animals. They tested this new method on 130 goat blood samples and 160 sheep blood samples, finding that it was very effective, with the best test for goats showing nearly perfect accuracy and a strong performance for sheep as well. This new test could be a reliable option for diagnosing CLA in goats and sheep.
Abstract
Caseous lymphadenitis (CLA) is a small ruminant disease characterized by the development of granulomatous lesions in superficial and internal lymph nodes, as well as in some organs, and causes significant economic losses worldwide. The aetiological agent of CLA is the bacterium Corynebacterium pseudotuberculosis; however, the commercially available diagnostic tools present problems with regard to specificity, which can lead to false-negative results. This study aimed to develop an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulins in goats and sheep using recombinant C. pseudotuberculosis PLD, CP40, PknG, DtxR and Grx proteins. For validation of the ELISAs, 130 goat serum samples and 160 sheep serum samples were used. The best ELISA for goats was developed using a combination of PLD and CP40 as antigens at a 1:1 ratio, which presented 96.9% sensitivity and 98.4% specificity. The most effective ELISA for sheep presented 91% sensitivity and 98.7% specificity when recombinant PLD alone was used as the antigen. These ELISAs can be used as highly accurate tools in epidemiological surveys and for the serodiagnosis of C. pseudotuberculosis infection in goats and sheep.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31287241/