A pilot study of a novel portable mass spectrometer for rapid, simultaneous detection of multiple anesthetic drug concentrations.

Abstract

In the perioperative management of emergency animals, anesthetic drug-related toxicity and adverse effects pose substantial risks, and real-time quantification of anesthetic drug concentrations may provide an effective strategy for improving anesthetic safety. This study aimed to evaluate the feasibility and accuracy of a novel Cell portable mass spectrometer (MS) for the rapid detection of multiple anesthetic drug concentrations. Etomidate (ET, 1.5&#x202f;mg/kg), rocuronium bromide (ROC, 5&#x202f;mg/kg), and lidocaine (LID, 2&#x202f;mg/kg) were administered, and plasma samples from rats were analyzed using the Cell portable MS and compared with results obtained by high-performance liquid chromatography with mass spectrometry (HPLC-MS). The Cell portable MS enabled simultaneous quantitative analysis of all three anesthetic drugs within 4.5&#x202f;min and demonstrated good linearity across detection ranges. The regression equations were y&#x202f;=&#x202f;0.01496x&#x202f;+&#x202f;0.3136 for ET (&#x202f;=&#x202f;0.997), y&#x202f;=&#x202f;1,356.03x&#x202f;+&#x202f;12,785.42 for ROC (&#x202f;=&#x202f;0.991), and y&#x202f;=&#x202f;0.01459x&#x202f;+&#x202f;0.0067 for LID (&#x202f;=&#x202f;0.999). Pearson correlation analysis revealed strong correlations between Cell portable MS and HPLC-MS measurements for all three drugs (ET:&#x202f;=&#x202f;0.9666; ROC:&#x202f;=&#x202f;0.9858; LID:&#x202f;=&#x202f;0.9937; all&#x202f;<&#x202f;0.0001). Bland-Altman analysis showed small mean biases, with most data points falling within the 95% limits of agreement and no proportional bias observed. Overall, the Cell portable MS demonstrated good quantitative agreement with conventional HPLC-MS for rapid, simultaneous measurement of multiple anesthetic drug concentrations, supporting its potential for real-time anesthetic drug toxicity monitoring and individualized dosing, particularly in emergency settings.