Peer-reviewed veterinary case report
Detecting Brucella canis in female dogs using vaginal swabs and PCR
By Keid, L B et al.·Published in Theriogenology·2007·Departamento de Medicina Veteriná, Brazil·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: A polymerase chain reaction for detection of Brucella canis in vaginal swabs of naturally infected bitches.
- Species:
- dog
Plain-English summary
A group of 144 female dogs with reproductive issues were tested for Brucella canis, a bacteria that can cause serious health problems. Researchers compared different testing methods, including a new PCR test done on vaginal swabs, to see how well they worked. The PCR test was able to detect the bacteria even in very small amounts and was particularly useful for dogs that tested negative in other tests. This new method could help identify infected dogs that might otherwise go unnoticed, which is important for controlling the spread of this disease.
People also search for: dog reproductive problems · Brucella canis test for dogs · dog vaginal swab PCR test
Abstract
A PCR assay for the detection of Brucella canis in canine vaginal swab samples was evaluated, comparing its performance with that of bacterial isolation, serological tests, and a blood PCR assay. One hundred and forty-four female dogs were clinically examined to detect reproductive problems and they were tested by the rapid slide agglutination test, with and without 2-mercaptoethanol (2ME-RSAT and RSAT, respectively). In addition, microbiological culture and PCR were performed on blood and vaginal swab samples. The results of the vaginal swab PCR were compared to those of the other tests using the Kappa coefficient and McNemar test. Of the 144 females that were examined, 66 (45.8%) were RSAT positive, 23 (15.9%) were 2ME-RSAT positive, 49 (34.02%) were blood culture positive, 6 (4.1%) were vaginal swab culture positive, 54 (37.5%) were blood PCR positive, 52 (36.2%) were vaginal swab PCR positive, and 50.69% (73/144) were positive by the combined PCR. The PCR was able to detect as few as 3.8 fg of B. canis DNA experimentally diluted in 54 ng of canine DNA, extracted from vaginal swab samples of non-infected bitches. In addition, the PCR assay amplified B. canis genetic sequences from vaginal swab samples containing 1.0 x 10(0) cfu/mL. In conclusion, vaginal swab PCR was a good candidate as a confirmatory test for brucellosis diagnosis in bitches suspected to be infected, especially those negative on blood culture or blood PCR; these animals may be important reservoirs of infection and could complicate attempts to eradicate the disease in confined populations.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/17920673/