A Quantitative Model for Estimating the Scale of Photochemically Induced Ischemic Stroke.

Abstract

Photothrombosis is a technique that can induce ischemic cortical infarcts using the photodynamic effect of anionic xanthene dyes, typically Rose Bengal, to cause occlusion of cerebral blood circulation. The ability to quantitatively predict the scale of the lesion in photothrombotic procedures can offer crucial insight in the development and implementation of light-induced stroke models in animals. In this article, we introduced a quantitative model that could estimate the normalized light intensity distribution in tissue which scatters photons from a collimated beam. We simulated the penetration and scattering profile of light of Rose Bengal's characteristic absorption wavelengths in mouse cortex. We further illustrated that our model could estimate the spatial extent of effective region under photothrombotic protocols, and how this model can be used to titrate the intensity and geometry of light beams used to generate infarcts of desired dimensional characteristics.