Peer-reviewed veterinary case report
A rapid assay for detecting antibody against Bluetongue virus with a latex agglutination test using recombinant VP7 antigen.
- Journal:
- Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
- Year:
- 2010
- Authors:
- Yang, Junxing et al.
- Affiliation:
- No. 2049 · China
Abstract
A latex agglutination test (LAT) for detecting antibody against Bluetongue virus (BTV) in ruminants was developed using latex beads coupled with recombinant VP7 protein. Compared with competitive enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the LAT were 99.0% and 93.0%, respectively. There was excellent agreement between the results obtained by competitive ELISA and the LAT (kappa = 0.930). Because it is rapid and easy to use, the LAT could be used for BTV antibody detection, especially for screening many serum samples.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/20224084/