Peer-reviewed veterinary case report
A rapid method for establishment of a reverse genetics system for canine parvovirus.
- Journal:
- Virus genes
- Year:
- 2017
- Authors:
- Yu, Yongle et al.
- Affiliation:
- Department of Biochemistry and Molecular Biology · China
- Species:
- dog
Abstract
Canine parvovirus (CPV) is an important and highly prevalent pathogen of dogs that causes acute hemorrhagic enteritis disease. Here, we describe a rapid method for the construction and characterization of a full-length infectious clone (rCPV) of CPV. Feline kidney (F81) cells were transfected with rCPV incorporating an engineered EcoR I site that served as a genetic marker. The rescued virus was indistinguishable from that of wild-type virus in its biological properties.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/28808849/