Peer-reviewed veterinary case report
A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples.
- Journal:
- The Journal of veterinary medical science
- Year:
- 2021
- Authors:
- Matsumoto, Fumi et al.
- Affiliation:
- Joint Faculty of Veterinary Medicine · Japan
Abstract
CD20 and CD3 are considered reliable markers for B and T cells, respectively. This study aimed to develop a rapid multiple immunofluorescence (RMIF) method for the detection of CD20 and CD3 on a single cytology slide. Air-dried smears were prepared using samples collected from dogs (n=26) and cats (n=6). Immunosignal detection using the newly developed method required 60 min. Clear immunosignals for CD20 and CD3 were detected in 24 of 26 samples in dogs and in all 6 cats. As the RMIF (CD20/CD3) method can detect markers of both B and T cells simultaneously on a single cytology smear, it would be an efficient tool for the immunophenotyping of canine and feline lymphoma samples.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/33814519/