Peer-reviewed veterinary case report
A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C.
- Journal:
- Journal of veterinary science
- Year:
- 2014
- Authors:
- Ding, Yao-Zhong et al.
- Affiliation:
- Lanzhou Veterinary Research Institute · China
Abstract
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/24690607/