Peer-reviewed veterinary case report
Fast test for bovine viral diarrhea virus in cattle
By Fan, Qing et al.·Published in Journal of virological methods·2012·Department of Biotechnology, China·View original on PubMed →
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Original publication title: A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus.
Plain-English summary
Researchers have developed a new test called RT-LAMP that can quickly and accurately detect the bovine viral diarrhea virus (BVDV) in cows. This test is very sensitive, able to find tiny amounts of the virus, and it works on both types of BVDV without confusing it with other bovine viruses. In a study of 88 fecal samples from cows, the RT-LAMP test identified 38 samples as positive for the virus, which is similar to the results from another established test. Overall, the RT-LAMP method shows promise as a fast and reliable way to diagnose BVDV in cows.
Abstract
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67×10(0)copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were found to be positive by RT-LAMP assay, whereas 39 were positive by real-time RT-PCR. Taken together, the BVDV specific RT-LAMP method is highly specific and sensitive and can be used as a rapid and direct diagnostic assay for testing clinical samples.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/22947692/