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Peer-reviewed veterinary case report

A Sensitive Autoradiographic Procedure for Factor VIII‐Related Antigen in Canine Plasma

Journal:
Veterinary Clinical Pathology
Year:
1983
Authors:
Schlink, Gerald T. & Johnson, Gary S.
Species:
dog

Abstract

SummaryA second‐antibody autoradiographic technique was found to be suitable for visualization of precipitin arcs produced by the elec‐troimmunoassay or crossed immunoelectro‐phoresis of factor Vlll‐related antigen in canine plasma. With the new autoradiographic procedure the electroimmunoassay was approximately ten times more sensitive than with the commonly used protein stain, Co‐omassie Blue R 250. Unlike earlier autoradiographic techniques, second‐antibody au‐toradiography requires only commercially available reagents.

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Original publication: https://doi.org/10.1111/j.1939-165x.1983.tb00618.x