Peer-reviewed veterinary case report
A simplified mycobacterial growth inhibition assay (MGIA) using direct infection of mouse splenocytes and the MGIT system.
- Journal:
- Journal of microbiological methods
- Year:
- 2016
- Authors:
- Yang, Amy L et al.
- Affiliation:
- Center for Biologics Evaluation and Research · United States
- Species:
- rodent
Abstract
We describe a simplified Mycobacterial Growth Inhibition Assay (MGIA) for pre-clinical assessment of vaccine-mediated protection in mice. The assay is accomplished by directly infecting splenocytes from vaccinated mice with Mycobacterium tuberculosis and quantifying mycobacteria using Mycobacterial Growth Indicator Tubes (MGIT). Vaccine-mediated immunogenicity detected by this assay correlated with protection.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/27650198/