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Peer-reviewed veterinary case report

A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).

Journal:
Journal of bacteriology
Year:
2007
Authors:
Sun, Boguang et al.
Affiliation:
Department of Marine Biology · China

Abstract

Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/17220231/