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Peer-reviewed veterinary case report

A small heat shock protein of Ostertagia ostertagi: stage-specific expression, heat inducibility, and protection trial.

Journal:
The Journal of parasitology
Year:
2006
Authors:
Vercauteren, Isabel et al.
Affiliation:
Faculty of Veterinary Medicine

Abstract

In this study, we isolated and analyzed a small heat shock protein (HSP) of Ostertagia ostertagi (Oo-HSP18). Oo-hsp18 is encoded by a single-copy gene and the full-length cDNA represents an 18-kDa protein. The expression of Oo-hsp18 is highly stage specific and restricted to the adult stage. The protein is synthesized in a tissue-specific manner and localized in the body muscle layer. The levels of Oo-hsp18 mRNAs are sharply induced by heat shock but not by other stressors such as levamisole and H2O2. A vaccination trial with recombinant Oo-HSP18 failed to protect calves against a challenge infection.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/17304801/