Peer-reviewed veterinary case report
Mutations in the c-kit gene found in 32 dogs with mast cell tumors
By Riva, Federica et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2005·Dipartimento di Patologia Animale, Italy·View original on PubMed →
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Original publication title: A study of mutations in the c-kit gene of 32 dogs with mastocytoma.
- Species:
- dog
Plain-English summary
A group of 32 dogs with mast cell tumors (mastocytoma) had their tumor tissue tested for changes in a specific gene called c-kit. Researchers found two types of mutations in the tumors, which could help understand how these tumors develop. The mutations were present in dogs with lower-grade tumors, but not in healthy dogs. This research may lead to better ways to diagnose and treat mast cell tumors in dogs.
People also search for: dog mast cell tumor treatment · c-kit gene mutations in dogs · symptoms of mastocytoma in dogs
Abstract
Mutations in the intracellular juxtamembrane domain of the c-kit gene in 32 dogs with different grades of histologically confirmed mastocytoma were studied. Transcript RNAs extracted from neoplastic tissue surgically collected from dogs of different breeds and from a negative control were reverse transcribed into complementary DNA and amplified by polymerase chain reaction. The region corresponding to the c-kit juxtamembrane domain was sequenced and compared with GenBank sequences. Two different types of mutations were identified within exon 11: a previously underscribed single-nucleotide substitution and a 6-bp deletion. The c-kit juxtamembrane domain sequences of all dogs were grouped in 3 clusters. No mutations were detected in tissues constitutively expressing c-kit (cerebellum and spleen), obtained from dogs not affected by mastocytoma (controls). All the substitutions were found in dogs bearing grade I or II mast cell tumors; the deletion was detected in 1 dog with grade II mastocytoma.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/16131001/