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Peer-reviewed veterinary case report

A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method.

Journal:
Journal of fish biology
Year:
2009
Authors:
Baker, D W et al.
Affiliation:
Department of Zoology · Canada

Abstract

This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).

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Original publication: https://pubmed.ncbi.nlm.nih.gov/20738495/