Peer-reviewed veterinary case report
AAV-Mediated Somatic Gene Editing for Cardiac and Skeletal Muscle in a Large Animal Model.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2022
- Authors:
- Ziegler, Tilman et al.
- Affiliation:
- Klinikum rechts der Isar der Technical University Munich · Germany
Abstract
Here we describe a protocol to produce a recombinant adeno-associated viral vector (rAAV)-based system to deliver the CRISPR-Cas9 complex into porcine skeletal muscle and myocardial cells. We initially describe the genomic composition of the rAAV-CRISPR vectors used in our lab. Furthermore, we give a step-by-step instruction into the production of recombinant viral vectors with high yields and purity. Lastly we describe the minimally invasive injection regimes to target the myocardium in a pig.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/36040587/