Abstracts from the 2022 Veterinary Dental Forum, September 28th – October 1st, Reno-Tahoe, Nevada, USA

Abstract

Periodontal disease is one of the most prevalent diseases in humans, dogs, and cats, imposing a signi fi cant burden on human and animal health. Control options remain nonspe-ci fi c, time-consuming, and costly; largely relying on the removal of dental plaque and calculus by mechanical debridement. However, while this reduces the formation of a disease-triggering dysbiotic bio fi lm, it does not directly affect the latent dysregulated in fl ammatory cascade in sus-ceptible hosts. Consequently, mechanical debridement requires constant repetition and provides variable prognoses. This has led to the indiscriminate use of antibiotics as an adjunct therapy. This injudicious use of antibiotics is alarm-ing, especially considering the ubiquity of periodontal disease and the systemic administration of antibiotics, as this contributes to the emergence and spread of world-wide antimicrobial resistance. mRNA vaccine platform with a microneedle-mediated deliv-ery to the buccal tissues. Our approach offers a fl exible platform for periodontal vaccines and may lead to veterinary and human medical applications due to interspecies similari-ties in periodontal disease. Molecular discoveries are revolutionizing our understanding of oral tumors in animals, and the notion of One Health has become even more relevant as parallels with human disease are revealed. Genomic approaches based on next generation sequencing technologies have become one of the main pillars of biomedical research and represent a critical element of the concept of personalized medicine. This lecture will review how genomic technologies are being used to reveal the molecular pathogenesis of the most common oral epithelial tumors in dogs, with a focus on current and future translatable diagnostic and therapeutic applications. Speci fi cally, recent molecular discoveries with the potential to translate into novel clinical interventions will be explained including how the mutational status of canine acanthomatous ameloblastoma and canine oral squamous cell carcinoma, as well as their comparative transcriptional pro fi les can inform possible druggable pathways. Possible future research directions will be mentioned. the cellular components; cytokine, and surface protein pro fi les; systemic effects, and transcriptome analysis of affected patients will be discussed. Patellar fracture and dental anomaly syndrome (PADS) in cats is characterized by atraumatic skeletal fractures, persistent deciduous teeth, and unerupted permanent teeth. Osteomyelitis of the jaws is a potential complication and often the reason that cats with this condition are presented for veterinary dental care. There are many unanswered ques-tions regarding the pathogenesis, prognosis, and best clinical management of these patients. I have been involved with evaluation of both oral and skeletal pathology of affected PADS cats since 2016. A manuscript summarizing the oral pathology fi ndings will be published in the Journal of Veterinary Dentistry and we are continuing to investigate the skeletal pathology and genetic etiology. This presentation summarizes key fi ndings to date. case study of utilizing Complete cell cell follow-up oral of Objective: To report the use of auricular cartilage grafts and fascia lata autologous membranes in the repair of challenging hard palate defects. Animals: Four dogs with a congenital midline hard palate cleft, two dogs with an acquired hard palate defect caudal to the incisive papilla, and one cat with a large acquired hard palate defect of unknown etiology were selected in this case series. All defects had subjectively either high risk of dehiscence if usual reconstructive techniques were employed or would have needed prior tooth extraction to use buccal mucosa fl aps for defect closure. Procedures: The palate defects were repaired by fi rst placing an auricular cartilage graft (17 × 27 mm to 60 × 30 mm) or a fascia lata autologous membrane (>30 × 50 mm) as a fi rst layer covered by two palatal fl aps ( fi ve dogs) and bilateral buccal advancement fl aps (one cat). In another dog, the graft was placed under the palatal mucosa, and the defect was left to heal by second intention. Results: Complete closure of the hard palate defects was achieved in fi ve cases (71.4%), and resolution of clinical signs occurred in all patients (100%). Necrosis of the apex of the ear (cat) and postoperative nasal discharge (one dog) were the only complications. Conclusion: The addition of a barrier made of auricular cartilage or fascia lata may be indicated to repair hard palate defects with no need of staging the procedure or to facilitate the healing of areas with high-risk of wound dehiscence.