Acetonic Fraction ofEnriched for Maturase K Is Able to Control Cerebral Parasite Burden in Mice Experimentally Infected With.

Abstract

infection can cause abortions or congenital infection for a vast number of domestic animals and humans, leading to economic loss in veterinary sciences, as well as severe consequences for immunocompromised patients.Linné has been used in ethnopharmacology for treatment of diseases, as malaria, diabetes and hepatitis, in addition to its use as antioxidant, antiallergic, anti-inflammatory, and antiviral. The components of this plant have never been studied before for treatment of toxoplasmosis, and the conventional drugs currently used to treat this disease have high degree of toxicity. Thus, the aim of this study was to evaluate the effect ofagainst, by analyzing a total extract of this plant in parallel with a fraction obtained by precipitation in acetone. Also, it was assessed if the acetonic fraction could present lectinic activity, followed by its identification by mass spectrometry. It was observed with the experimental models designed that both total extract and acetonic fraction ofwere able to controlinfection byandexperiments, in addition to their low toxicity to host cells. Both total extract and acetonic fraction of this plant display capacity to impair replication oftachyzoites. Interesting, theacetonic fraction treatment for 10 days after infection decreases significantly the number ofbrain cyst in comparison with controls. The protein isolated fromacetonic fraction was characterized as a novel lectin identified as maturase K. Taken together, these findings open new perspectives to treat patients infected by. Future studies will be necessary to investigate the precise mechanism underlying the control ofinfection to impair the replication of this parasite in the host cells after treatment withmaturase K.