Peer-reviewed veterinary case report
Active Induction of Experimental Autoimmune Encephalomyelitis (EAE) with MOGin the Mouse.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2018
- Authors:
- Giralt, Mercedes et al.
- Affiliation:
- Institute of Neurosciences · Spain
- Species:
- rodent
Abstract
Experimental autoimmune encephalomyelitis (EAE) is one of the most popular animal models of multiple sclerosis (MS). There are a number of EAE models, being actively induced EAE in strains such as C57Bl/6 mice very robust and reproducible. We herewith present details of the materials and methods for active EAE. Mice are immunized with an emulsion of myelin oligodendrocyte glycoprotein peptide 35-55 (MOG) + complete Freund's adjuvant (supplemented with Mycobacterium tuberculosis), and treated with Bordetella pertussis toxin, to induce EAE. Sham-EAE mice are immunized with bovine serum albumin instead of MOG.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/30006713/