Peer-reviewed veterinary case report
Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections.
- Journal:
- Nature methods
- Year:
- 2018
- Authors:
- Mlodzianoski, Michael J et al.
- Affiliation:
- Weldon School of Biomedical Engineering · United States
Abstract
Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-μm-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-β filaments in a mouse model of Alzheimer's disease.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/30013047/