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Peer-reviewed veterinary case report

ADAMTS-18 in the host tissues exerts little effect on breast tumor progress in a murine 4T1 breast cancer model.

Journal:
Journal of negative results in biomedicine
Year:
2016
Authors:
Liu, Min et al.
Affiliation:
Key Laboratory of Brain Functional Genomics (East China Normal University) · China
Species:
rodent

Abstract

BACKGROUND: In this study, we aimed to identify a novel extracellular proteinase ADAMTS-18 that could be a potential tumor suppressor gene. RESULTS: We successfully constructed Adamts-18 knockout mice with BALB / c background. RT-PCR analysis showed syngeneic mammary tumor cell line 4 T1 per se has weakly endogenous ADAMTS-18 expression. Orthotopic inoculation of 4 T1 cells within the mammary fat pad of host mice, we found no significant difference in tumor growth and metastasis between Adamts-18 knockout mice and widetype control. CONCLUSIONS: We did not confirm that ADAMTS-18 in the host tissues is relevant for breast tumor progress in a murine 4 T1 breast cancer model.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/26841794/