Peer-reviewed veterinary case report
Adaptation of three Babesia divergens isolates to continuous culture in rat erythrocytes.
- Journal:
- Journal of the Egyptian Society of Parasitology
- Year:
- 2004
- Authors:
- Musa, Najla B & Abdel Gawad, Mahrashan A
- Affiliation:
- Department of Medical Parasitology
- Species:
- rodent
Abstract
Three Babesia divergens isolates have been cultured continuously for 6 months in rat erythrocytes using the candle jar technique. One isolate was already rat-adapted, the other two became adapted to rats through continuous culturing in rat erythrocytes. Babesia was cultured in rat erythrocytes in RPMI medium supplemented with 20% foetal calf serum. The highest parasitaemia was 35% and multi-parasitization of red blood cells was often observed. Cultures of B. divergens remained infective to splenectomized rats. Cultures with high parasitaemias contained a large number of extra-cellular merozoites, when separated from the red blood cells, they retained their infectivity.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/15125537/