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Peer-reviewed veterinary case report

Adenovirus-mediated expression of BmK CT suppresses growth and invasion of rat C6 glioma cells.

Journal:
Biotechnology letters
Year:
2013
Authors:
Du, Jun et al.
Affiliation:
Institute of Biotechnology · China
Species:
rodent

Abstract

BmK CT, one of the key toxins in the venom of the scorpion, Buthus martensii Karsch, can interact specifically with glioma cells as a chloride channel blocker and inhibit the invasion and migration of those cells via MMP-2. A recombinant adenovirus, Ad-BmK CT, was constructed and characterized by in vitro and in vivo studies, using MTT cytotoxicity assay and the glioma C6/RFP (red fluorescence protein)/BALB/c allogeneic athymic nude mice model, respectively. The adenovirus-mediated expression of BmK CT displayed a high activity in suppressing rat C6 glioma cells growth and invasion thereby suggesting that this recombinant adenovirus may be a powerful method for treating glioblastoma.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/23443213/