Aerosolic Application of Phages Against <i>S. infantis</i> on Plates and Chicken Skin.

Abstract

Phages are known as a promising method to combat antimicrobial resistance (AMR) in the human and veterinary sector. Use of phage aerosols enormously increases the application field, although the impact on the infectivity of phages during nebulization needs to be evaluated. In this study <i>S. infantis</i> was treated on plates and chicken skin with nebulized phage particles of the <i>Myoviridae</i> type, identified by transmission electron microscopy, using a commercial nebulizer primarily used for H₂O₂ disinfection. The reduction of bacterial number by aerosol applied phage particles was evaluated. It could clearly be shown that the phage particles were able to infect <i>Salmonella</i> after being nebulized using ultrasound technology. Further studies on other types of phages as well as other conditions must be performed to standardize the aerosolic application of phages.