Peer-reviewed veterinary case report
African Swine Fever Virus Hemadsorption Inhibition Assay.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2022
- Authors:
- Malogolovkin, Alexander & Sereda, Alexey
- Affiliation:
- Federal Research Center for Virology and Microbiology (FRCVM)
Abstract
Adsorption of red blood cells (hemadsorption) on the surface of macrophages infected with African swine fever virus (ASFV) is a unique phenomenon allowing to determine virus infectious titer in hemadsorption unit (HAU) and differentiate virus strains phenotypically. In the meantime, hemadsorption of particular ASFV strain can by inhibited by homologous anti-ASFV serum containing antibody to the serogroup-specific virus protein (CD2v). Here, we describe a hemadsorption inhibition assay (HADIA) to phenotype ASFV strains to one of the known nine serogroups using blood-derived swine macrophages. The HADIA is a powerful method in the ASFV immunopathology and vaccine research since it provides additional antigenic and phenotypic characteristics of virus strains that can't be defined by other assays.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/35575893/