Ameliorative effects ofvar.ethanol extract and fractions on cimetidine-induced reproductive toxicity in male Wistar rats.

Abstract

BACKGROUND: Male infertility is a global health issue, with pharmaceutical agents such as cimetidine contributing significantly to gonadotoxicity through antiandrogenic and oxidative mechanisms. The search for natural protective agents has highlightedvar.(collard greens) for its antioxidant and endocrine-modulating properties. OBJECTIVES: This study evaluated the protective effects ofvar.(collard greens) ethanol extract and its solvent fractions on cimetidine-induced reproductive toxicity in male Wistar rats, focusing on body/organ weights, hormonal profiles, antioxidant enzyme activities, and testicular histoarchitecture. METHODS: Thirty-five rats were divided into seven groups: control, cimetidine (120&#x202f;mg/kg), ethanol extract (200&#x202f;mg/kg), and cimetidine&#x202f;+&#x202f;fractions (aqueous, butanol, and hexane). After 8&#x202f;weeks oral administration of extracts/fractions, sperm parameters, serum hormones (LH, FSH, and testosterone), oxidative stress markers (catalase, SOD, and MDA), and histopathology were assessed. RESULTS: Cimetidine significantly reduce follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone levels (<&#x202f;0.05) while increasing oxidative stress, as evidenced by elevate malondialdehyde (MDA) superoxide dismutase (SOD) and catalase (CAT). It also caused distortion of the testicular architecture. Treatment with the ethanol extract (ELEBO) and solvent fractions restored hormonal balance and antioxidant activity. Histological analysis revealed preserved testicular architecture in treated groups compared to the degeneration observed in the cimetidine-induced group. CONCLUSION: var.exhibits significant protective effects against cimetidine-induced reproductive toxicity through hormonal regulation, antioxidative mechanisms, and tissue preservation. The ethanol extract of(ELEBO) showed the most potent activity, supporting its potential use as a therapeutic adjunct in male infertility linked to pharmaceutical exposures.