Peer-reviewed veterinary case report
-Aminobutyric acid enhances myogenesis and heat/cold stress resistance in bovine muscle satellite cells.
- Journal:
- Frontiers in veterinary science
- Year:
- 2026
- Authors:
- Manzoor, Abid et al.
- Affiliation:
- Department of Animal Science · China
Abstract
INTRODUCTION: -Aminobutyric acid (GABA) is the principal inhibitory neurotransmitter in the central nervous system and is involved in the development of neural tissue as well as the regulation of its functions. Meanwhile, GABA has also been demonstrated to confer multiple physiological benefits, including alleviating stress and improving metabolic homeostasis. This study investigated GABA effects on proliferation, differentiation, and temperature stress protection of bovine skeletal muscle satellite cells (BSCs). METHODS: BSCs were exposed to graded GABA concentrations for 24-96 h; based on CCK-8 and EdU assays, 0.5 and 1 mM were selected for mechanistic experiments (RT-qPCR, Western blot, immunofluorescence). We further evaluated GABA under heat (41 °C) and cold (4 °C) stress by measuring stress-related genes, antioxidant indices, Nrf2/Keap1/HO-1 pathway proteins, and by performing transcriptomic analysis. RESULTS: GABA significantly promoted BSC proliferation ( < 0.05) by upregulating,,,, andmRNA, and enhanced myogenic differentiation ( < 0.05) by increasing MyoG, MyoD, and MyHC protein levels, with generally stronger effects at 0.5 mM. Under 41 °C, GABA reduced,,, andmRNA ( < 0.05), whereas at 4 °C it increased,, andmRNA and lowered intracellular ROS ( < 0.05). At 41 °C, 0.5 mM GABA elevated HO-1 protein ( < 0.05); at 4 °C, it upregulated,, andmRNA, while HO-1 protein declined ( < 0.05). Transcriptomics revealed enrichment of the p53 and PI3K-Akt pathways at 41 °C and the p53 and FoxO pathways at 4 °C (key nodes:,,,,,,,). In anrumen-protected GABA (RP-GABA) trial under different THI conditions, RP-GABA significantly increased body length at Temperature Humidity Index (THI) 45 and THI 63 (< 0.05). CONCLUSION: Across timepoints and indices, 0.5 mM GABA provided protection comparable to or better than 1 mM, suggesting 0.5 mM as the recommended comprehensive doseand offering molecular targets for functional feeds to improve ruminant resilience to temperature stress.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/42058559/