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Peer-reviewed veterinary case report

An efficient single prime protocol for the induction of antigen-induced airways inflammation.

Journal:
Journal of immunological methods
Year:
2013
Authors:
Shaw, Odette M & Harper, Jacquie L
Affiliation:
Malaghan Institute of Medical Research
Species:
rodent

Abstract

There are a number of mouse models of allergic airway inflammation used to delineate various aspects of asthma. One of the hurdles with using mice is their natural resistance to developing a Th2 allergic response. This is often overcome by double priming with the allergen and an adjuvant. Here we report on an efficient 11day, single antigen/alum priming protocol that is sufficient to sensitise mice for the development of Th2-mediated inflammation in the lung following antigen challenge.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/23831610/