An experimental model of fetal growth restriction based on selective ligature of uteroplacental vessels in the pregnant rabbit.

Abstract

INTRODUCTION: To describe an animal model of growth restriction based on selective ligature of uteroplacental vessels in the pregnant rabbit. MATERIAL AND METHODS: Two experimental protocols (+21 and +25 days of gestation) with three groups were defined: controls, mild (20-30%) and severe (40-50%) uteroplacental vessel ligature. Fetuses were delivered 120 h after the procedure by cesarean section. Biometrical measurements were carried out. Brains were obtained and glial response and cell proliferation were studied by S100beta and Ki-67 immunohistochemistry. RESULTS: Mortality rate and biometrical restriction increased across experimental groups according to the time and severity of the procedure. S100beta expression was significantly higher in the severe reduction group at 25 days. Ki-67 expression was significantly higher in the mild reduction group at 21 days and in the severe reduction group at 25 days. DISCUSSION: Selective ligature of uteroplacental vessels in the pregnant rabbit results in a gradual model of growth restriction in terms of mortality, biometrical restriction and histological brain changes.