Peer-reviewed veterinary case report
An explant based-method for differentiating adipocytes from equine adipose tissue.
- Journal:
- Equine veterinary journal
- Year:
- 2013
- Authors:
- Suagee, J K & Corl, B A
- Affiliation:
- Department of Dairy Science · United States
- Species:
- horse
Abstract
Culturing adipocytes enables fine control of experimental conditions and helps minimise animal use. This report describes an explant-based method for isolating stromal-vascular cells from equine adipose tissue that enables use of small amounts of tissue. Subcutaneous and mesenteric adipose tissues were harvested post mortem and stromal-vascular cells grown from explants, prior to testing the capacity of several differentiation media to induce lipid droplet formation and increase transcript abundance of adipocyte markers. Inclusion of rosiglitazone at 1 and 5 µmol/l concentrations, along with other media components, induced differentiation of cultured equine stromal-vascular cells derived from subcutaneous and mesenteric adipose tissues.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22607277/