An in vitro model for discovery of osteoclast specific biomarkers towards identification of racehorses at risk for catastrophic fractures.

Abstract

BACKGROUND: Focal bone microcracks with osteoclast recruitment and bone lysis, may reduce fracture resistance in racehorses. As current imaging does not detect all horses at risk for fracture, the discovery of novel serum biomarkers of bone resorption or osteoclast activity could potentially address this unmet clinical need. The biology of equine osteoclasts on their natural substrate, equine bone, has never been studied in vitro and may permit identification of specific biomarkers of their activity. OBJECTIVES: (1) Establish osteoclast cultures on equine bone, (2) Measure biomarkers (tartrate resistant acid phosphatase isoform 5b [TRACP-5b] and C-terminal telopeptide of type I collagen [CTX-I]) in vitro and (3) Study the effects of inflammation. STUDY DESIGN: In vitro experiments. METHODS: Haematopoietic stem cells, from five equine sternal bone marrow aspirates, were differentiated into osteoclasts and cultured either alone or on equine bone slices, with or without a pro-inflammatory stimulus (IL-1&#x3b2; or LPS). CTX-I and TRACP-5b were immunoassayed in the media. Osteoclast numbers and bone resorption area were assessed. RESULTS: TRACP-5b increased over time in osteoclast cultures without bone (p&#x2009;<&#x2009;0.0001) and correlated with osteoclast number (r&#xa0;=&#xa0;0.63, p&#x2009;<&#x2009;0.001). CTX-I and TRACP-5b increased with time for cultures with bone (p&#xa0;=&#xa0;0.002; p&#xa0;=&#xa0;0.02 respectively), correlated with each other (r&#xa0;=&#xa0;0.64, p&#x2009;<&#x2009;0.002) and correlated with bone resorption (r&#xa0;=&#xa0;0.85, p&#x2009;<&#x2009;0.001; r&#xa0;=&#xa0;0.82, p&#x2009;<&#x2009;0.001 respectively). Inflammation had no measurable effects. MAIN LIMITATIONS: Specimen numbers limited. CONCLUSIONS: Equine osteoclasts were successfully cultured on equine bone slices and their bone resorption quantified. TRACP-5b was shown to be a biomarker of equine osteoclast number and bone resorption for the first time; CTX-I was also confirmed to be a biomarker of equine bone resorption in vitro. This robust equine specific in vitro assay will help the study of osteoclast biology.