Peer-reviewed veterinary case report
An Orthotopic Implantation Mouse Model of Hepatocellular Carcinoma with Underlying Liver Steatosis.
- Journal:
- STAR protocols
- Year:
- 2020
- Authors:
- Kasashima, Hiroaki et al.
- Affiliation:
- Department of Pathology and Laboratory Medicine · United States
Abstract
This protocol provides the steps required for a mouse liver orthotopic implantation model. The reliable pre-clinical animal models that have similar characteristics to hepatocellular carcinoma (HCC) are a powerful tool to unveil the mechanisms controlling tumor initiation and progression. Here, we describe a syngeneic orthotopic HCC model that recapitulates the role of a host pro-tumorigenic microenvironment by pre-conditioning mouse livers with a high-fat diet (HFD). For complete details on the use and execution of this protocol, please refer to Kudo et al. (2020).
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/33377079/